Fang Zhiyuan,Ph.D, Master'sSupervisor

E-mail:fangjnu@126.com

Personal brief introduction:

His research focuses on two directions.（1）Novel technology for diseases, toxic and harmful substances detection; monoclonal antibody production.

（2）Medical Devices for tumor diagnosis and treatment.He has published over 40 research papers in peer-reviewed international journals, such as 《Advanced Science》、《Biosensors and Bioelectronic》、《Chemical Communications》. He also published obtained 10 patents and two scientific research projects (one National Natural Science Foundation and one provincial natural science Foundation)

Education background：

2009.9-2012.6：Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Ph.D, Biochemistry and Molecular Biology

2006.9-2009.7：Ji’nan University,Postgraduate, Immunology

2002.9-2006.7：Ji’nan University,Biomedical engineering

Work experience：

2019.12-today：Associate professor, Guangdong University of Technology

2017.10-2019.11：Associate Researcher, The Fifth Affiliated Hospital of Guangzhou Medical University

2016.3-2017.9：Associate researcher, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences

2013.12-2015.12：Postdoctoral fellow, The Affiliated Cancer Hospital of Guangzhou Medical University

2012.7-2013.10：research assistant/Associate researcher, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences

Publications：

1)Dou Wang, Chenchen Ge, Weiyuan Liang, Qinhe Yang, Quan Liu, Wei Ma, Lulin Shi,Hong Wu, Yuhua Zhang, Zongze Wu, Chaoying Wei, Luodan Huang, Zhiyuan Fang,*Liping Liu,* Shiyun Bao,* and Han Zhang*.In Vivo Enrichment and Elimination of Circulating Tumor Cells by Using a Black Phosphorus and Antibody Functionalized Intravenous Catheter. Advanced Science, 2020,6. DOI:10.1002/advs.202000940.

2)Ning Sun, Dou Wang, Guoqiang Yao, Xiaomei Li, Ting Mei, Xinke Zhou, Kwok-Yin Wong, Baishan Jiang*, Zhiyuan Fang*. pH dependent and cathepsin B activable CaCO3 nanoprobe for targeted in vivo tumor imaging. International Journal of Nanomedicine, 2019:14 1–9.

3)Wei Wu, Ning Sun, Simin Zhu, Xiaomei Li, Jitao Chen, Chunzhao Liu, Zhiyuan Fang* and Qingli Yang*. Ultrasensitive strips for the quadruple detection of nitrofuran metabolite residues. RSC Adv., 2019,9,2812-2815.

4)Zhiyuan Fang, Sichao Zheng, Kin-FaiChan*, Wenchang Yuana, Qi Guo, Wenyu Wu, Hoi-KuiLui, Yujing Lu, Yun-Chung Leung, Tak-Hang Chan, Kwok-Yin Wong*, NingSun*. Design, synthesis and antibacterial evaluation of 2,4-disubstituted-6-thiophenyl-pyrimidines. Eur J Med Chem. 2019,161:141-153.

5)Xuewen Lu, Ting mei, Qi Guo, Wenjing Zhou, Xiaomei Li, Jitao Chen, Xinke Zhou*, Ning Sun*, Zhiyuan Fang*. Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles. Microchimica Acta. 2018, 4;186(1):2.

6)Wei Wu*, Meng Zhou, Hong He, Chunzhao Liu, Peifeng Li, Man Wang, Ying Liu, Xiaodan Haoa, Zhiyuan Fang*. A sensitive aptasensor for the detection of Vibrio parahaemolyticus. Sensors & Actuators: B. Chemical. 2018,272 (2018) 550–558.

7)Ting mei, Xuewen Lu, Ning Sun, Xiaomei Li, Jitao Chen, Min Liang, Xinke Zhou, Zhiyuan Fang*. Real-time quantitative PCR detection of circulating tumor cells using tag DNA mediated signal amplification strategy. Journal of Pharmaceutical and Biomedical Analysis. 2018, 158 (2018) 204–208.

8)Zhiyuan Fang, Li Ban, Yanan Li, Wenchang Yuan, Zhihua Liu, Ting Liu, Xiaomei Li, Kwok-yin Wong, Yujing Lu, Ning Sun, Xingang Yao. A quinoline-based FtsZ inhibitor for the study of antimicrobial activity and synergistic effects with β-lactam antibiotics. J Pharmacol Sci. 2018. 137(3):283-289.

9)Ning Sun, Qi Guo, Xiaomei Li, Jitao Chen, Xiaowen Liu, Kwok-Yin Wong* and Zhiyuang Fang*. An isothermal single base extension based lateral flow biosensor and electrochemical assay for gene point mutation detection. Analytical Methods. 2018, 10, 2863-2868.

10)Wei Wu*, Lin Wang, Yang Liu, Dou Wang, Tulong Huang, Baishan Jiang and Zhiyuan Fang*. Site-specific DNA methylation detection based on enzyme-linked immunosorbent assay using recombinant methyl-CpG binding protein. Clin Chem Lab Med. 2018, 56 (7):e164.

11)Lingwen Zeng, Zhiyuan Fang and Yunbo Wang. Colorimetric Detection of Copper Ion Based on Click Chemistr. dx.doi.org/10.5772/intechopen.76024.

12)Tingyu Yu, Jiao Lin, Jin Zhao, Wei Huang, Lingwen Zeng, Zhiyuan Fang *, Ning Xu *. A simple in vitro tumor chemosensitivity assay based on cell penetrating peptide tagged luciferase. Plos one. 2017,12(11):e0186184.

13)Zhiyuan Fang, Yu Wang, Hao Li, Shuaishuai Yu, Ziying Liu, Zhichao Fan, Xiaomin Chen, Yuying Wu, Xuebo Pan, Xiaokun Li, and Cong Wang. Combination treatment of citral potentiates the efficacy of hyperthermic intraperitoneal chemoperfusion with pirarubicin for colorectal cancer. Mol Pharm. 2017,14(10):3588-3597.

14)Xuewen Lu, Xiaoling Liang, Jianghong Dong, Zhiyuan Fang*, Lingwen Zeng*. Lateral flow biosensor for multiplex detection of nitrofuran metabolites based on functionalized magnetic beads. Anal Bioanal Chem. 2016 Jul 20. 408(24):6703-9（

15)Xiaoling Liang, Lin Wang, Dou Wang, Lingwen Zeng* and Zhiyuan Fang*. Portable and quantitative monitoring of mercury ions using DNA-gated mesoporous silica nanoparticles using a glucometer readout. Chemical Communications. 2016, DOI: 10.1039/C5CC08611F. 52(10):2192-4.

16)Ruan Qiang, Fang Zhiyuan, Cui Shuzhong*, Zhang Xiangliang, Wu Yinbing, Tang Hongsheng, Tu Yinuo, Ding Yan. Thermo-chemotherapy Induced miR-218 upregulation inhibits the invasion of gastric cancer via targeting Gli2 and E-cadherin. Tumor Biol. 2015, 36(8):5807-14.

17)Wei Wu, Zhiyuan Fang, Shiming Zhao, Xuewen Lu, Luxin Yu, Ting Mei, Lingwen Zeng*. A simple aptamer biosensor for Salmonellae enteritidis based on fluorescence-switch signaling graphene oxide. RSC Advances, 2014, 4, 42, 22009.

18)Huang Baotian, Fang Zhiyuan, Huang Y, Lin P, Chen Z*. A dosimetric analysis of volumetric-modulated arc radiotherapy with jaw width restriction vs 7 field intensity-modulated radiotherapy for definitive treatment of cervical cancer. The British journal of radiology. 2014, 87(1039):20140183.

19)Zhiyuan Fang, Wei Wu, Xuewen Lu, Lingwen Zeng*. Lateral flow biosensor for DNA extraction-free detection of salmonella based on aptamer mediated strand displacement amplification. Biosensors and Bioelectronics. 2014, 15(56) 192-197.

20)Zhiyuan Fang, Baishan Jiang, Wei Wu, Zhicheng Xiang, Chuanyan Ouyang, tulong Huang, Junhua Chen and Lingwen Zeng*. ELISA detection of semicarbazide based on a fast sample pretreatment method. Chemical Communications. 2013, 49(55):6164-6.

21)Zhiyuan Fang, Wenjuan Zhang, Chenchen Ge, Jie Liu, Puchang Lie, and Lingwen Zeng*. A sandwich assay for quantitative detection of transcription factor in cell lysate. Analyst. 2012, 137 (18), 4127 - 4130.

22)Zhiyuan Fang, Chenchen Ge, Wenjuan Zhang, Puchang Lie, Lingwen Zeng*. A lateral flow biosensor for rapid detection of DNA-binding protein c-jun. Biosensors and Bioelectronics. 2011, (27) 192-196.

23)Zhiyuan Fang, Jing Huang, Puchang Lie, Zhuo Xiao, Chuanyan Ouyang, Qing Wu, Yixing Wu, Guodong Liu and Lingwen Zeng*. Lateral flow nucleic acid biosensor for Cu2+ detection in aqueous solution with high sensitivity and selectivity. Chemical Communications. 2010, 46, 9043-9045.

24)Zhiyuan Fang, Feiyue Xing*, Bronner C, et al. ICBP90 mediates the ERK1/2 signaling to regulate the proliferation of Jurkat T cells. Cellular Immunology. 2009; 257(1-2):80-7.

25)方志远; 邢飞跃*; 郭中锋; 陈玲. Jagged-1对骨髓来源树突状细胞生成细胞因子的影响。细胞与分子免疫学杂志，2009-02-18。

26)方志远; 邢飞跃*. Jagged 1促进肿瘤发生。生物学杂志，2008-12-18。

27)方志远; 邢飞跃*. Jagged-1在肿瘤新生血管形成中的双重作用。中国肿瘤生物治疗杂志，2008-04-30。

28)Junhua Chen, Zhiyuan Fang, Puchang Lie, and Lingwen Zeng*. Computational lateral flow biosensor for proteins and small molecules: A new class of strip logic gates. Anal. Chem. 2012, 84, 6321-6325.

29)Chenchen Ge, Zhiyuan Fang, Junhua Chen, Jie Liu, Xuewen Lu and Lingwen Zeng*. A simple colorimetric detection of DNA methylation. Analyst. 2012, 137, 2032-2035.

30)Junhua Chen, Zhiyuan Fang, Jie Liu, Lingwen Zeng*. A Simple and rapid biosensor for ochratoxin A based on a structure-switching signaling sptamer. Food Control. 2012,25, 555-560.

31)Wu W, Yu L, Fang Z, Lie P, Zeng L*. A lateral flow biosensor for the detection of human pluripotent stem cells. Anal Biochem. 2013 Feb 12. doi:pii: S0003-2697(13)00063-8. 10.1016/j.ab.2013.01.034.

32)Liu J, Chen J, Fang Z, Zeng L*. A simple and sensitive sensor for rapid detection of sulfide anions using DNA-templated copper nanoparticles as fluorescent probes. Analyst. 2012 Dec 7;137(23):5502-5. doi: 10.1039/c2an35885a.

33)Xiao Z, Lie P, Fang Z, Yu L, Chen J, Liu J, Ge C, Zhou X, Zeng L*. A lateral flow biosensor for detection of single nucleotide polymorphism by circular strand displacement reaction. Chem Commun. 2012 Sep 4;48(68):8547-9. doi: 10.1039/c2cc33931e.

34)Chen J, Liu J, Fang Z, Zeng L*. Random dsDNA-templated formation of copper nanoparticles as novel fluorescence probes for label-free lead ions detection. Chem Commun. 2012 Jan 25;48(7):1057-9. doi: 10.1039/c2cc16668b.

35)Lie P, Liu J, Fang Z, Dun B, Zeng L*. A lateral flow biosensor for detection of nucleic acids with high sensitivity and selectivity. Chem Commun. 2012 Jan 7;48(2):236-8. doi: 10.1039/c1cc15878c.

36)Wu W, Chen J, Fang Z, Ge C, Xiang Z, Ouyang C, Lie P, Xiao Z, Yu L, Wang L, Zeng L*. A self-assembled deoxyribonucleic acid concatemer for sensitive detection of single nucleotide polymorphism. Anal Chim Acta. 2013 Dec 4; 804:235-9. doi: 10.1016/j.aca.2013.09.055.

37)Yu L, Wu W, Chen J, Xiao Z, Ge C, Lie P, Fang Z, Chen L, Zhang Y, Zeng L*. Spectrophotometric and ultrasensitive DNA bioassay by circular-strand displacement polymerization reaction. Analyst. 2013 Dec 7;138(23):7182-7. doi: 10.1039/c3an01347b. Epub 2013 Oct 15.

38)Ge C, Yu L, Fang Z, Zeng L*. An enhanced strip biosensor for rapid and sensitive detection of histone methylation. Anal Chem. 2013 Oct 1;85(19):9343-9. doi: 10.1021/ac402202x. Epub 2013 Sep 11.

39)Ge C, Chen J, Wu W, Fang Z, Chen L, Liu Q, Wang L, Xing X, Zeng L*. An enzyme-free and label-free assay for copper(II) ion detection based on self-assembled DNA concatamers and Sybr Green I. Analyst. 2013 Sep 7;138(17):4737-40. doi: 10.1039/c3an00973d. Epub 2013 Jul.

40)Wu W, Zhao S, Mao Y, Fang Z, Lu X, Zeng L*. A sensitive lateral flow biosensor for Escherichia coli O157:H7 detection based on aptamer mediated strand displacement amplification. Anal Chim Acta. 2015 Feb 25; 861:62-8.

41)Zhang XL, Shi HJ, Wang JP, Tang HS, Wu YB, Fang ZY, Cui SZ, Wang LT*. MicroRNA-218 is upregulated in gastric cancer after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy and increases chemosensitivity to cisplatin. World J Gastroenterol. 2014 Aug 28;20(32):11347-55.